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denuding tools

Denuding Pipettes

Description / Use

SLM Denuding Pipettes are used with SLM 3-in-One Pipette controller for denudation and gametes and embryo transfer.

The pipettes are made of borosilicate glass which is known for its stability and is resistant to corrosion and remains absolutely neutral, even to aggressive chemicals in nearly all fields of chemistry.

  • The glass pipette has better wetting feature comparing to plastic one and is less likely to have oil drops attached inside which can sometimes damage an oocyte and embryo.
  • Unpolished pipettes are used to remove cumulus cells attached to the zona prior to micro manipulation procedures and fertilization assessment.
  • Polished pipettes are used to transfer blastomere (during PGS,) oocytes and embryos from one dish to another during other assisted reproduction procedures.
Catalogue NO. Inner Diameter Tip Style
SPD-120 120-124 Un-polished
SPD-125 125-129 Un-polished
SPD-130 130-134 Un-polished
SPD-140 135-144 Un-polished
SPD-70P 65-74 Polished
SPD-80P 75-84 Polished
SPD-90P 85-99 Polished
Catalogue NO. Inner Diameter Tip Style
SPD-70P 65-74 Polished
SPD-80P 75-84 Polished
SPD-90P 85-99 Polished
SPD-100P 100-119 Polished
SPD-120P 120-139 Polished
SPD-140P 140-159 Polished
SPD-160P 160-189 Polished
SPD-190P 190-219 Polished
SPD-220P 220-249 Polished
SPD-250P 250-289 Polished
SPD-290P 290-319 Polished
SPD-320P 320-360 Polished

pzd pipettes

PZD Pipettes

Description / Use

  • SLM PZD Pipettes are used to cut an opening on the zona pellucida of an oocyte mechanically to enable assisted hatching and/or embryo biopsy.
  • The pipette is designed with a very sharp tip and a strong taper section for easy control and precision manipulation.
  • They are supplied with 0°-45° tip angle and a tip to elbow length of 0.55mm.
Catalogue No. Description Tip Angle Degree
SPD-30 Solid sharp tip 30°

Instructions For Use:

  • To open the case, hold the case and make sure that “Sunlight” mark is up facing your right hand.
  • Using your left index finger and thumb press the case on both side marked “IIIII” then using your right index finger and thumb pull the top cover.
  • Hold firmly the pipette in the middle and gently pull it out from the base.

blastomere biopsy pipettes

Blastomere Biopsy Pipettes

Description / Use

  • SLM Blastomere Biopsy Pipettes are used to remove blastomeres and trophectoderm cells from embryos for pre-implantation genetic diagnosis(PGD).
  • There are five sizes and two series available for selection. The ‘Z’ series is flat tip while the ‘X’ series is beveled tip. Both styles are supplied with an ID in 20&#xB5m, 25&#xB5m, 30&#xB5m, 35&#xB5m, and 40&#xB5m. Size 20&#xB5m and 25&#xB5m are recommended for trophectoderm (TE) biopsy.
  • There is no shrinkage at the bending section, so less likely to damage blastomere in case it is sucked through.
  • All tipsare fire polished to reduce damage to the cell membrane.
  • They are supplied with a tip angle of 0°-45° and a tip to elbow length of 0.55mm.
Catalogue No. Tip Style Inner Diameter(&#xB5m) Tip Angle Degree
SBB-20Z-xx Flat 20∓0.2 xx-0° – 45°
SBB-25Z-xx Flat 25∓0.2 xx-0° – 45°
SBB-30Z-xx Flat 30∓0.2 xx-0° – 45°
SBB-35Z-xx Flat 35∓0.2 xx-0° – 45°
SBB-40Z-xx Flat 40∓0.2 xx-0° – 45°
SBB-20X-xx Beveled 20∓0.2 xx-0° – 45°
SBB-25X-xx Beveled 25∓0.2 xx-0° – 45°
SBB-30X-xx Beveled 30∓0.2 xx-0° – 45°
SBB-35X-xx Beveled 35∓0.2 xx-0° – 45°
SBB-40X-xx Beveled 40∓0.2 xx-0° – 45°

polar body biopsy pipettes

Polar Body Biopsy Pipettes

Description / Use

  • SLM Polar Body Biopsy Pipettes are used to remove polar bodies from human oocytes and embryos for pre-implantation genetic diagnosis(PGD).
  • There are three series of pipettes available for selection: 15z (flat tip), 15x (beveled tip) and 15s (spiked tip).
  • All these pipettes are designed with a fire polished edge to avoid damage to the cell membrane.
  • They are supplied with a tip angle of 0° – 45° and a tip to elbow length of 0.55mm.
Catalogue No. Inner Diameter(µm) Tip Style Tip Angle Degree
SPB-15Z-xx 13-15 Flat 20°, 25°, 30°, 35°
SPB-15X-xx 13-15 Beveled 20°, 25°, 30°, 35°
SPB-15S-xx 13-15 Beveled & Spiked 20°, 25°, 30°, 35°

zona drilling pipettes

Zona Drilling Pipettes

Description / Use

  • SLM Zona Drilling Pipettes are used to apply acidic solution for creating a hole on the zona pellucida to enable assisted hatching and/or embryo biopsy.
  • There are two sizes available for selection:ID8-10µm and ID:10-12µm. These pipettes are designed with a slightly fire polished tip.
  • They are supplied with 0° -45° tip angle and a tip to elbow lenght of 0.55mm.
Catalogue No. Inner Diameter(µm) Tip Style Tip Angle Degree
SZD-10-xx 8-10 Flat 20°, 25°, 30°, 35°
SZD-12-xx 10-12 Flat 20°, 25°, 30°, 35°

holding pipettes

Holding Pipettes

Description / Use

  • SLM Holding Pipettes are used to hold an oocyte or embryo in position during ICSI and other micro-manipulation procedures.
  • There are three series and six sizes for selection. The classic series has an ID 15-20µm and OD in 90µm, 100µm,120µm and 130µm. The ‘S’ series has smaller ID(10-15µm). The ‘B’ series has an ID 25-30µm and OD in 120µm, 130µm and 1180µm. The ‘B’ series may by used for holding an oocyte with cumulus cells attached and for embryo biopsy.
  • All our holding pipettes are supplied with a tip angle of 0° to 45° and a tip elbow length of 0.65mm.
  • Packed in a box of 10.

Ordering Information

Catalogue No. Outer Diameter(µm) Inner Diameter(µm) Tip Angle Degree
SHP-70S-xx 70∓5 10-15 xx-0° to 45°
SHP-90-xx 90∓5 15-20 xx-0° to 45°
SHP-100-xx 100∓5 15-20 xx-0° to 45°
SHP-120-xx 120∓5 15-20 xx-0° to 45°
SHP-120B-xx 120∓5 25-30 xx-0° to 45°
SHP-130-xx 130∓5 15-20 xx-0° to 45°
SHP-130B-xx 130∓5 25-30 xx-0° to 45°
SHP-180B-xx 175∓5 25-30 xx-0° to 45°

icsi tools

ICSI Injection Pipettes

Description / Use

  • SLM ICSI Injection Pipettes are used to perform intracytoplasmic sperm injection (ICSI).
  • SLM offers three types of spiked and two types of non-spiked ICSI INJECTION PIPETTES.
  • SLM offers three sizes of ICSI Injection Pipette.
  • All our injection pipettes are supplied with an angle of 0° – 45° and a tip to elbow length of 0.55 mm and 1.0 mm.
Catalogue No. Size Tip Style Tip to Elbow Length Tip Angle Degree
SIC-45V-xx
SIC-45V-xxL
SIC-45W-xx
SIC-45W-xxL
SIC-45H-xx		 ID:4.0-4.5µm
OD:5-6µm		 Spiked tip xx-0.55mm
xxL-1.0mm		 xx-0° to 45°
SIC-45T-xx
SIC-45T-xxL
SIC-45N-xx
SIC-45N-xxL		 ID:4.0-4.5µm
OD:5-µm		 Non Spiked tip xx-0.55mm
xxL-1.0mm		 xx-0° to 45°
SIC-50V-xx
SIC-50V-xxL
SIC-50W-xx
SIC-50W-xxL
SIC-50H-xx		 ID:4.5-5.0µm
OD:6-7µm		 Spiked tip xx-0.55mm
xxL-1.0mm		 xx-0° to 45°
SIC-55V-xx
SIC-55V-xxL
SIC-55W-xx
SIC-55W-xxL
SIC-55H-xx		 ID:5.0-5.5µm
OD:6-7µm		 Spiked tip xx-0.55mm
xxL-1.0mm		 xx-0° to 45°

sysm iii b

SYMS III

Description / Use

The SYMS III sealer is a bench top unit that can be used under laminar rlow hood and is using the “thermal” procedure to weld CBS High Security 0.3mL, 0.5mL and 2mL straws, CBS High Security Tubes as well as the HSV High Security Vitrification Straw.

Technical Specifications

Setup, maintenance and downtime time are approximately 5 minutes per day.

Potentially contaminated parts of the system can be cleaned with ethanol or non-corrosive decontamination fluids.

L x W x H: 363 x 276 x 152mm
Weight: 6kg
Power Supply: 110 or 220V/60 or 50Hz

    Key Features

  • Available in 3 languages (French, English or Spanish).
  • Glove compatible touch screen interface.
  • Easy-to-use touch screen advises user of the machines actions:
    heating up, ready, welding process, etc.
  • Pre-set specific combination of seal temperature, time and jaw position for each CBS device.
  • Two sealing modes: fully automatic straw detection system or touch screen operator control.
  • Error management: alarm message on touch screen and audible signal.
  • Electronic touch screen monitors and advises on the maintenance needs of the machine.
  • Easy to clean and maintain.

    Instructions For Use

  • Place the CBS High Security straw of the CBS High Security tube holder;
  • Place the straw or tube on the holder and gently push forward;
  • Automatic mode: As soon as the sensor detects the straw or tube in the sealing position, the sealing process starts automatically.
  • Manual mode: the straw or tube is sealed when the operator presses the ‘WELD’ button on touch screen.
Reference Description Packaging
010287 CBS™ High Security sperm straw 0.3 mL, White cotton plug 4 cells of 5 straws
010288 CBS™ High Security sperm straw 0.3 mL, White cotton plug 4 cells of 5 straws
014651 CBS™ High Security sperm straw 0.5 mL, White cotton plug 4 cells of 5 straws
014650 CBS™ High Security sperm straw 0.5 mL, Coloured cotton plug external part: white 5 cells of 20 straws
016584 CBS™ High Security sperm straw 0.5 mL, Coloured cotton plug external part: Red 5 cells of 20 straws
016611 CBS™ High Security sperm straw 0.5 mL, Coloured cotton plug external part: Yellow 5 cells of 20 straws
016612 CBS™ High Security sperm straw 0.5 mL, Coloured cotton plug external part: Green 5 cells of 20 straws
016613 CBS™ High Security sperm straw 0.5 mL, Coloured cotton plug external part: Blue 5 cells of 20 straws
016614 CBS™ High Security sperm straw 0.5 mL, Coloured cotton plug external part: Orange 5 cells of 20 straws
016615 CBS™ High Security sperm straw 0.5 mL, Coloured cotton plug external part: Gray 5 cells of 20 straws
007426 Recisperme with tube – Disposable sterile device for sperm collection  
015937 Isolating transport container for sperm tubes – Mix of colours  

storage liquid nitrogen

Storage In Liquid Nitrogen

Description / Use

Cryo Bio System carries a line of accessories for storage and management of HSV kits in liquid nitrogen.

    Thawing

    Thawing of the specimen should be done in a controlled environment, such as a laminar flow hood.

  • Prepare the thawing/dilution media
  • Identify the HSV straw
  • Transfer the straw from the storage container to a transport dewar filled with liquid nitrogen.
  • Lift the straw enough to expose the colored handling rod. Make sure the end with the sample remains immersed in the liquid nitrogen.
  • While holding the straw, use the opening device for HSV kit to section the straw.
  • Immediately (within 2 seconds), plunge the gutter into the first dilution media.

    Shelf-Life

  • Three years from date of manufacture.

embryo freeze thaw media

Embryo Freeze Thaw Media

Description / Use

Embryo Freeze and Thaw Media kits consist of convenient, ready-to-use solutions designed for the cryopreservation and recovery of cleavage-stage embryos (zygote to 8-cell).

The composition of these solutions is based upon published standard propanediol and sucrose frezing (2 step) and thawing (3 step) protocols, which have proven to be the most clinically successful methods for cryopreservation and recovery of zygote to early cleavage stage human embryos, achieving similar implantation and pregnancy rates to that for transfers of fresh embryos.

Irvine Scientific has performed extensive efficacy testing on our Embryo Freeze and Thaw Media using mouse embryos and two different bioassay methods. Since standard MEA (exposure only) does not specifically emulate the intended application of these products; and as part of our ongoing efforts to enhance the sensitivity of our QC test methods, a specific Embryo Recovery Assay was developed and used to emulate the intended use of these products.

These solutions were also evaluated using the standard one-cell mouse embryo assay, typically employed as the accepted QC text method for commercially available formulas.

Independent IVF laboratories showed that the recovery of one-cell cryopreserved mouseembryos from Irvine Scientific’s Embryo Freeze and Thaw Media was equivalent to, or better than that achieved for their control cryopreservation media (in house prepared media using either propanediol / sucrose or DMSI methods) currently used in clinical practice for cryopreservation of human embryos.

Use of the embryo Recovery Assay (with one-cell mouse embryos) is a highly sensitive performance test for the intended use of Irvine Scientific’s Embryo Freeze and Thaw Media. High blastocyst development rates were obtained using the standard mouse embryo assay. Which confirm the lack of toxicity of these solutions. The use of these media can achieve up to 100% recovery of cryopreserved cleavage-stage embryos.

    Embryo Freeze Media Kit

  • Embryo Freeze Medium-F1,2x10mL
  • Embryo Freeze Medium-F1,4x10mL

    Embryo Thaw Media Kit

  • Embryo Thaw Medium-T1,2x10mL
  • Embryo Thaw Medium-T2,2x10mL
  • Embryo Thaw Medium-T2,2x10mL

    Features And Benefits

  • Convenient ready-to-use kits consisting of two media for cryopreservation and three media for the thawing of embryos.
  • Rapid protocol with just two steps to freeze and thee steps to thaw.
  • Each solution contains HTF medium buffered with HEPES to maintain proper pH during cryopreservation and thawing procedures at ambient temperature and atmosphere.
  • Gentamicin sulfate is included in each medium as an antibiotic.
  • Store at 2-8℃.
  • Shelf life of 6 months.
Reference Packaging
90116 6x10mL
90124 6x10mL