Cryo Bio System

continuous single culture hsa

Continuous Single Culture® Complete with HSA

Description / Use

Continuous Single Culture® Complete product (CSCM-C) with Human Serum Albumin is a novel solution designed for fertilization and embryo culture through day 5/6 of embryo development. This medium is optimized to be used in an uninterrupted culture system without dish change or medium renewal.

Pre-supplemented with HSA for a final total protein concentration of 5 mg/mL.

Quality Control Testing:

Available in 2x20mL configuration.
Store at 2-8℃.

Reference	Description
90165	2x20ml CSC Complete

continuous single culture

Continuous Single Culture

Description / Use

Culture media for human gametes and embryos during fertilization and growth of embryos through Day 5/6 of development.

Continuous Single Culture

Available in 60 mL configuration.
Shelf life is 90 days from date of manufacture.
Shelf life after opening is 8 weeks.
Contains Gentamicin as the antibiotic.
Requires protein supplementation.

Continuous Single Culture Complete

Available in 2×20 mL Kits.
Shelf life is 120 days from date of manufacture.
Ready to use, pre-supplemented with Human Serum Albumin (10% v/v HSA), for a final total protein concentration of 5mg/mL.

Features And Benefits

Continuous Single Culture Media is the only true continuous culture medium that is clinically proven to improve implantation rates over a sequential media system.

For use from fertilization through Day 5/6 of development.
Optimized for use in a continuous culture system, without the need to refresh medium.
Only one medium to order, inventory and store.

Reference	Description	Packaging
90165	CSC Complete	2×20 ml

continuous single culture nx

Continuous Single Culture®- NX

Description / Use

Continuous Single Culture® – NX (CSCM-NX) is a clinically-proven, low lactate, single-step culture medium that helps improves blastocyst development. CSCM-NXC is designed for fertilization and embryo culture through day 5/6 of embryo development. This medium is optimized to be used in an uninterrupted culture system, without dish change or medium renewal.

Quality Control Testing:

Storage : 2-8℃.
Shelf Life : 120 days
Gentamicin.
In a M-199 HEPES Buffered Medium.

Reference	Description
90165	2x20ml CSC Complete

oocyte collection

Oocyte Collection Needle

Description / Use

The devices feature:

An echogenic triple bevel single lumen needle.
A thin tube connecting the needle to a bung.
A vacuum tube connecting the bung to the aspiration source.
A three-way flushing valve (for the model concerned), to choose between aspiration or rinsing the follicles.

Reference	Description	Packaging
1301020	OPS® Classic without valve	Box of 10 units
1301021	OPS® Classic with valve	Box of 10 units

ops

OPS

Description / Use

A single channel needle, triple-beveled, with a 5 mm grooved echogenic zone before the beveled tip.
A thin tubing connecting the needle to a bung. The latter is fitted onto a Falcon® type collecting tube.
A three-way valve (for the model concerned), to choose between aspiration or rinsing of the follicles.
A vacuum tube connecting the bung to the aspiration source.

The complete sets (needle + tubing) provide adapted storage conditions for the oocytes during the collection, particularly being able to maintain the vials at an appropriate temperature using heating systems. The resilient properties of polyurethane ensure flexibility without kinking for a regular flow rate, with no loss of suction. The specific quality of steel and polishing techniques improve the needle’s handling and sliding motion. The grooved circular markings provide echogenicity very close to the bevel.

Reference	Description	Packaging
1313101	OPS® ART of CCD® without valve	Box of 10 units

vit kit thaw

Vit Kit®-Thaw

Description / Use

Vitrification Thaw Kit is for use in the thawing of vitrified oocytes (MII), pronuclear (PN) zygotes through day 3 cleavage stage embryos and blastocyst stage embryos.

Each kit contains liquid and ready-to-use solutions. It does not require CO2 incubation.

4 Vials of TS (Thawing Solution, 4 x 2mL). Contains

1 M sucrose.
20% DSS.
Gentamicin.
In a M-199 HEPES Buffered Medium.

1 Vial of DS (Dilution Solution, 1 x 2mL). Contains

0.5 M sucrose.
20% DSS.
Gentamicin.
In a M-199 HEPES Buffered Medium.

1 Vial of WS (Washing Solution, 1 x 2mL). Contains

20% DSS.
Gentamicin.
In a M-199 HEPES Buffered Medium.

Reference	Description
90137	TS, 4x1ml
90137	DS, 1x1ml
90137	WS, 1x2ml

vit kit freeze

Vit Kit®-Freeze

Description / Use

Vitrification Freeze Kit is for use in the vitrification of oocytes (MII), pronuclear (PN) zygotes through day 3 cleavage stage embryos and blastocyst stage embryos.

Each kit contains liquid and ready-to-use solutions. It does not require CO₂ incubation.

2 Vials of ES (Equilibration Solution, 2 x 1 mL). Contains

7.5% DMSO.
7.5% ethylene glycol.
20% DSS.
Gentamicin.
In a M-199 HEPES Buffered Medium.

2 Vials of VS (Vitrification Solution, 2 x 1 mL). Contains

15% DMSO.
15% ethylene glycol.
20% DSS.
0.5 M sucrose.
Gentamicin.
In a M-199 HEPES Buffered Medium.

Reference	Description	Packaging
90133	ES, 1x1ml	10 Cryo Tips
90133	VS, 2x1ml	10 Cryo Tips

hsv straw red

HSV High Security Vitrification Straw – Red

Description / Use

High Security Vitrification (HSV) Red Straw (4 straws)

The only high security closed system for oocyte & embryo vitrification HSV straws provide the highest quality cryopreservation of biological samples.

Quality Control Testing:

Advanced Vitrification Techniques.
Vitrification and Warming of Blastocysts.
Pregnancy results following initiation of a blastocyst vitrification program.
Vitrification and warming of donor oocytes using a closed straw system.

Reference	Description	Packaging
025246	HSV High Security Vitrification kits – Red	Pack of 4
025247	HSV High Security Vitrification kits – White	Pack of 4
025248	HSV High Security Vitrification kits – Yellow	Pack of 4
025249	HSV High Security Vitrification kits – Purple	Pack of 4
025250	HSV High Security Vitrification kits – Blue	Pack of 4
025251	HSV High Security Vitrification kits – Green	Pack of 4

hyaluronidase solution

Hyaluronidase Solution

Description / Use

Hyaluronidase Solution is a convenient, ready-to-use solution designed for removing cumulus cells surrounding freshly retrieved oocytes prior to ICSI or other ART procedures.

This solution consists of 80 IU/mL of Hyaluronidase enzyme in a HEPES buffered HTF medium with therapeutic-grade human serum albumin (50mb/m:) and the antibiotic gentamicin sulfate (10µg/mL).

Irvine Scientific has performed extensive efficacy testing on Hyaluronidase Solution using freshly retrieved mouse oocytes and one-cell embryos.

The performance of Irvine Scientific’s Hyaluronidase Solution was equivalent to control hyaluronidase solutions, and no toxicity was observed to mouse oocytes and embryos. The development of one-cell embryos to blastocysts was equivalent after exposure to Hyaluronidase Solution as compared to non exposed controls (in growth medium only). The lot-to-lot performance of HJyaluronidase Solution was consistent for enzyme activity and the removal of cumulus cells from freshly retrieved oocytes with in 2 minutes.

In addition to the mouse oocyte and embryo bioassays, Hyaluronidase Solution is also tested for enzyme activity using a standard enzymatic assay for Hyaluronidase. This permits verification of actual enzyme activity, in addition to the functional bioassay of cumulus dell removal and lack of toxicity.

Morover, stability studies have been conducted to determine the enzymatic activity of Irvine Scientific’s Hyaluronidase Solution after storage at several temperatures. The results is greater than 95% of the initial enzymatic activity (80IU/mL) remains after storage for 2 months at 6℃ (refrigerated) or 6 months frozen (-10℃).

The recommended storage temperature for this product is frozen (<-10℃) to assure a shelf life of at least 6 months. Repeated freeze/thaw cycles of Hyaluronidase Solution (no more than 3) did not adversely affect the enzymatic activity.

Hyaluronidase Solution

Available in kits of 5×1.0mL.
The shelf life is 6 months when stored sealed at the recommended temperature of (<-10℃).
Gentamicin sulfate is included as the antibiotic.

Features And Benefits

Convenient ready-to-use solution for denuding oocytes of cumulus cells prior to ICSI or other ART procedures. Refer to product insert for detailed usage instructions.
This solution contains HEPES buffered HTF medium to maintain proper pH during cumulus cell removal at ambient atmosphere conditions.

Reference	Packaging
90101	5x1mL

modified htf medium

Modified HTF Medium with Gentamicin – HEPES

Description / Use

Modified HTF Medium is a synthetic, defined solution for procedures which include the retrieval, handling and transfer of human gametes and embryos. mHTF is composed of a HEPES and sodium bicarbonate buffering system, making it ideal for use in ambient atmosphere conditions. It requires protein supplementation before use.

Quality Control Testing:

Store at 2-8℃.
12 months from date of manufacture.

Reference	Description
90166	100ml
90166	500ml